Effects of Thymoquinone on Behavioral and Biological Alterations in an Experimental Rat Model of Alzheimer Disease /
Abulfadl, Yasmin Saad,
Effects of Thymoquinone on Behavioral and Biological Alterations in an Experimental Rat Model of Alzheimer Disease / تأثير الثايموكينون علي السلوك والتغيير الحيوي في النموذج التجريبي لمرض الزهايمر في الجرذان. Yasmin Saad Abulfadl ; Supervised by Amany AIi Eissa Ahmed, Osama Ahmed Badary, Nabila Nour Eldin EI-Maraghy. - 1 Volumes : illustrations ; 25 cm
Thesis (PH.D.)- Ain Shams University. Faculty of Pharmacy. Pharmaceutical Chemistry Department,2018.
Includes bibliographic references (p. 194 - 241).
neuroprotective effect of thymoquinone (TQ) on Alzheimer
disease (AD) pathogenesis by comparing the effect of three
different doses of(TQ) (10, 20,.40mg/kg) on AD rat model and on
normal animals. The study was further extended to illuminate the
mechanistic pathways influenced by TQ treatment.
AD experimental model was established in Sprague Dawley
male albino rats by intraperitoneal injection of both D-galactose
(D-gal) (60mg/kg) and aluminum chloride (AICI3) (lOmg/kg) for
42 days. After injection of D-gal and AICl3 for 28 days, the rats
were treated concomitantly with PO administration of TQ at 10,
20, 40 mg/kg/day doses levels once daily for consecutive 14 days
respectively.
Behavioral responses of animals were assessed in a Morris
water maze and passive avoidance tests. At the end of this period
all rats were anaesthetized and sacrificed by cervical dislocation.
Brains were carefully excised and divided sagittally. The left
hemibrains were immediately fixed in 4% PF A for histology and
immunohistochemistry. The right hemibrains were homogenized
for analyzing of biochemical changes by assessing oxidative stress
biomarkers such as nitric oxide (NO) and malondialdehyde
(MDA), anti-oxidant biomarkers such as total antioxidant capacity
(T AC) and superoxide dismutase, antiapoptotic biomarker such as
beta cel1 lymphoma-2 (Bcl-2), the specific proinflammatory
cytokines such as tumor necrosis factor (TNF-a.) and interleukin-
1 beta (lL-l~) and brain cholinergic biomarkers, such as
acetylcholinesterase (AChE) as well as assessment of brain
derived neurotrophic factor (BDNF). Then the study was further
extended to investigate the mechanistic pathways influenced by
TQ treatment by studying and understanding the key signal
The Text in English and the summaries in English and Arabic.
Drug development--Abbreviations
Drug development--Acronyms
Pharmaceutical industry--Abbreviations
Pharmaceutical industry--Acronyms
Drug Design--Abbreviations
Drug Design--Handbooks
Drug Industry--Abbreviations
Drug Industry--Handbooks
Pharmaceutical Preparations--Handbooks
Pharmaceutical Preparations--Abbreviations
615.19 / A.Y.E
Effects of Thymoquinone on Behavioral and Biological Alterations in an Experimental Rat Model of Alzheimer Disease / تأثير الثايموكينون علي السلوك والتغيير الحيوي في النموذج التجريبي لمرض الزهايمر في الجرذان. Yasmin Saad Abulfadl ; Supervised by Amany AIi Eissa Ahmed, Osama Ahmed Badary, Nabila Nour Eldin EI-Maraghy. - 1 Volumes : illustrations ; 25 cm
Thesis (PH.D.)- Ain Shams University. Faculty of Pharmacy. Pharmaceutical Chemistry Department,2018.
Includes bibliographic references (p. 194 - 241).
neuroprotective effect of thymoquinone (TQ) on Alzheimer
disease (AD) pathogenesis by comparing the effect of three
different doses of(TQ) (10, 20,.40mg/kg) on AD rat model and on
normal animals. The study was further extended to illuminate the
mechanistic pathways influenced by TQ treatment.
AD experimental model was established in Sprague Dawley
male albino rats by intraperitoneal injection of both D-galactose
(D-gal) (60mg/kg) and aluminum chloride (AICI3) (lOmg/kg) for
42 days. After injection of D-gal and AICl3 for 28 days, the rats
were treated concomitantly with PO administration of TQ at 10,
20, 40 mg/kg/day doses levels once daily for consecutive 14 days
respectively.
Behavioral responses of animals were assessed in a Morris
water maze and passive avoidance tests. At the end of this period
all rats were anaesthetized and sacrificed by cervical dislocation.
Brains were carefully excised and divided sagittally. The left
hemibrains were immediately fixed in 4% PF A for histology and
immunohistochemistry. The right hemibrains were homogenized
for analyzing of biochemical changes by assessing oxidative stress
biomarkers such as nitric oxide (NO) and malondialdehyde
(MDA), anti-oxidant biomarkers such as total antioxidant capacity
(T AC) and superoxide dismutase, antiapoptotic biomarker such as
beta cel1 lymphoma-2 (Bcl-2), the specific proinflammatory
cytokines such as tumor necrosis factor (TNF-a.) and interleukin-
1 beta (lL-l~) and brain cholinergic biomarkers, such as
acetylcholinesterase (AChE) as well as assessment of brain
derived neurotrophic factor (BDNF). Then the study was further
extended to investigate the mechanistic pathways influenced by
TQ treatment by studying and understanding the key signal
The Text in English and the summaries in English and Arabic.
Drug development--Abbreviations
Drug development--Acronyms
Pharmaceutical industry--Abbreviations
Pharmaceutical industry--Acronyms
Drug Design--Abbreviations
Drug Design--Handbooks
Drug Industry--Abbreviations
Drug Industry--Handbooks
Pharmaceutical Preparations--Handbooks
Pharmaceutical Preparations--Abbreviations
615.19 / A.Y.E