MARC details
| 000 -LEADER |
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| fixed length control field |
03731nam a2200289 i 4500 |
| 001 - CONTROL NUMBER |
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| control field |
22549624 |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20210926135451.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
901022s2015 ii a b 00110 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
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| International Standard Book Number |
9788123926605 |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
WYU |
| Transcribing agency |
WYU |
| Description conventions |
rda |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
|---|
| Edition number |
23 |
| Classification number |
574.1928 |
| Item number |
R.J.B |
| 100 10 - MAIN ENTRY--PERSONAL NAME |
|---|
| Personal name |
Robyt, John F., |
| Dates associated with a name |
1935- |
| Relator term |
author |
| 245 10 - TITLE STATEMENT |
|---|
| Title |
Biochemical techniques : |
| Remainder of title |
theory and practice / |
| Statement of responsibility, etc |
John F. Robyt, Bernard J. White. |
| 264 #1 - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
|---|
| Place of publication, distribution, etc |
New Delhi : |
| Name of publisher, distributor, etc |
CBS publishers & distributors, |
| Date of publication, distribution, etc |
2015. |
| 300 ## - PHYSICAL DESCRIPTION |
|---|
| Extent |
xvi, 407 pages : |
| Other physical details |
illustrations ; |
| Dimensions |
24 cm. |
| 336 ## - CONTENT TYPE |
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| Source |
rdacontent |
| Content type term |
text |
| 337 ## - MEDIA TYPE |
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| Source |
rdamedia |
| Media type term |
unmediated |
| 338 ## - CARRIER TYPE |
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| Source |
rdacarrier |
| Carrier type term |
volume |
| 500 ## - GENERAL NOTE |
|---|
| General note |
Reprint. Originally published: Monterey, Calif. : Brooks/Cole Pub. Co., 1987. |
| 500 ## - GENERAL NOTE |
|---|
| General note |
pharmacy bookfair2016 |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc |
Includes bibliographical references and index. |
| 520 ## - SUMMARY, ETC. |
|---|
| Summary, etc |
Biochemical Techniques presents the fundamental theory and practice used in biochemistry laboratory courses and in biochemical research. Chapter I covers the making of measurements and error analysis, the presentation of data in tables and graphs, and the writing of reports. Chapter 2 presents the preparation and properties of solutions and includes pH and buffers. For some students, the contents of Chapters I and 2 may already have been covered in other chemistry courses. Chapter 3 proceeds with qualitative and quantitative techniques including UV and visible spectrophotometry, fluorimetry, and infrared and nuclear magnetic resonance spectroscopy. Chapter 4 covers chromatographic methods of analysis and is divided into two parts. Part I discusses the general aspects of the many types of chromatography and Part II covers specific applications for separating carbohydrates, amino acids, proteins, nucleic acids, and lipids. Chapter 5 presents electrophoretic methods of separation and places emphasis on zone electrophoresis, especially polyacrylamide- and agarose-gel electrophoresis. Chapter 6 discusses the nature and properties of radioisotopes and techniques for quantitatively measuring radioactivity by gas-ionization, liquid and solid scintillation spectrometry, and autoradiography. Specific methods of radioisotope labeling of biochemical compounds and applications of their use in tracer experiments are presented. Chapter 6 concludes with a discussion of the points that should be considered in designing an experiment using radioisotopes. Chapter 7 gives various, commonly used methods for the qualitative and quantitative determination of carbohydrates, amino acids, proteins, nucleic acids, and lipids. Wherever possible we have given the underlying chemistry and the limitations of the methods. Chapter 8 presents the techniques used in the preparation of different kinds of biochemical substances and includes cell lyses, centrifugation, filtration, concentration, dialysis, and fermentation. Specific methods of preparing organelles, proteins, carbohydrates, nucleic acids, and lipids are given. Within each class, specific examples were chosen to illustrate different sources, methods, and biochemical properties. For example, in the protein class, the preparation of jack bean urease is described for historical reasons as well as for the simplicity of the method of crystallization; yeast alcohol dehydrogenase is chosen to illustrate the combination of several different types of techniques used in protein purification; human salivary o-amylase is presented because it can be readily obtained from a human source and because of its ease of assay and frequent use in studying enzyme catalysis in laboratory courses. |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
|---|
| Topical term or geographic name as entry element |
Biochemistry |
| General subdivision |
Technique. |
| 700 10 - ADDED ENTRY--PERSONAL NAME |
|---|
| Personal name |
White, Bernard J., |
| Dates associated with a name |
1937- |
| Relator term |
author |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
|---|
| Source of classification or shelving scheme |
Dewey Decimal Classification |
| Koha item type |
Books |