MARC details
| 000 -LEADER |
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| fixed length control field |
05096nam a22003617i 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION |
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| control field |
20241013160733.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
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| fixed length control field |
211026s2021 ua a|||| bm|| 00| 0 eng d |
| 040 ## - CATALOGING SOURCE |
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| Original cataloging agency |
EG-NcFUE |
| Description conventions |
rda |
| 041 0# - LANGUAGE CODE |
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| Language code of text/sound track or separate title |
eng |
| Language code of summary or abstract/overprinted title or subtitle |
ara |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
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| Edition number |
22 |
| Classification number |
615.32 |
| Item number |
F.N.C |
| 100 1# - MAIN ENTRY--PERSONAL NAME |
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| Personal name |
Fathi, Noha Mostafa Fathallah, |
| Relator term |
author. |
| 245 10 - TITLE STATEMENT |
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| Title |
Chemical and biological evaluation of metabolites isolated from fruits, wastes and endophytic fungi of cultivated Ammi majus L. plant / |
| Statement of responsibility, etc |
by Noha Mostafa Fathallah Fathi (Assistant Lecturer of Pharmacognosy and Medicinal Plants, Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Future University) |
| 246 15 - VARYING FORM OF TITLE |
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| Title proper/short title |
التقييم الكميائي والبيولوجي للمركبات المفصولة من الثمار و النفايات والفطريات الداخلية لنبات الخلة الرباني المنزرع |
| 264 #1 - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
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| Date of publication, distribution, etc |
2021 |
| 300 ## - PHYSICAL DESCRIPTION |
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| Extent |
1 online resource (236 pages, 4 pages) : |
| Other physical details |
illustrations (some color) |
| 336 ## - CONTENT TYPE |
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| Source |
rdacontent |
| Content type term |
text |
| 337 ## - MEDIA TYPE |
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| Source |
rdamedia |
| Media type term |
computer |
| 338 ## - CARRIER TYPE |
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| Source |
rdacarrier |
| Carrier type term |
online resource |
| 500 ## - GENERAL NOTE |
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| General note |
Supervision of Prof. Dr. Mostafa Aly Abd El Kawy (Professor of Pharmacognosy, Faculty of Pharmacy, Cairo University), Prof. Dr. Osama Mostafa Salama (Professor of Pharmacognosy, Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Vice President Future University, Egypt), Associate Prof. Marwa Yousry Mostafa Issa (Associate professor of Pharmacognosy, Faculty of Pharmacy, Cairo University, Egypt), Dr. Mokhtar Mohamed Bishr (Plant General Manager and Technical Director, Arab Company for Pharmaceuticals and Medicinal Plants, Egypt) |
| 502 ## - DISSERTATION NOTE |
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| Dissertation note |
Thesis (P.hD.)-Cairo university, Faculty of pharmacy, Department of Pharmacognosy, 2021. |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE |
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| Bibliography, etc |
Includes bibliographical references. |
| 520 3# - SUMMARY, ETC. |
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| Summary, etc |
Ammi majus L., family Apiaceae growing in Egypt is famous for comprising active constituents as coumarins, flavonoids, and steroids. Its main used part is the fruits that contain furanocoumarins widely used in skin conditions like vitiligo and psoriasis. Firstly: This study is meant to investigate the bioactive metabolites present in the aerial parts (waste) in comparison with that of the fruits and optimize the cultivation conditions to enhance the constituents present in the fruits. Optimization was via exposure to drought stress and applying an exogenous phytohormone (ethephon) then comparing the results with that of the control fruit group. The physical parameters besides the differences in the total phenolics, total flavonoid contents, and the antioxidant activity of the extracts were evaluated. Remarkably, the aerial parts revealed higher total phenolics and flavonoids with much stronger antioxidant activity than the fruit extract. The positive and negative metabolic profiling using UPLC/MS-MS of the aerial parts extract revealed different compounds when compared to the compounds present in the fruits. Secondly: The Ammi majus, fruits were investigated for their phytochemical compounds responsible for their pharmacological activities. The dried powdered plant was extracted with methanol, fractionated using gradient elution then the CH2Cl2 fraction was purified using puri-flash. The compounds isolated were identified using 1HNMR and 13CNMR as β-sitosterol, Isoarnottinin, Xanthotoxin, Marmesin, Imperatorin, and Ammirin. Thirdly: An endophytic fungus was isolated from the fruits and identified as Aspergillus amstelodami (MK215708). After mass production of the fungus on rice media, the active metabolites were extracted using EtOAc then fractionated and purified using preparative HPLC to give Dihydroauroglaucin, tetrahydroauroglaucin, 2-(3,6-dihydroxyhepta-1,4-dien1-yl)-3,6-dihydroxy-5-(dimethylallyl) benzaldehyde, isotetrahydroauroglaucin), flavoglaucin and, 2-(2, 3-epoxy-1,3,5- heptatrienyl)-6-hydroxy-5-(3-methyl-2-butenyl) benzaldehyde. Structure elucidation was carried out using (1 H-and 13 C-NMR, HMBC, HSQC, and COSY). Finally, Antimicrobial, Antibiofilm, cytocompatibility, and cytotoxicity assays were done based on biological guided technique. The antimicrobial and antibiofilm results revealed that the major fungus compound (dihydrauroglaucin) had the strongest activity followed by the fungus fractions, the FEA then the plant extract. The cytocompatibility effect of AME, FEA, and dihydrauroglaucin revealed no toxicity on the normal human fibroblastic cell line (BHK). For cytotoxicity against HepG2, the AME, FEA, their fractions, and the major plant compound xanthotoxin were investigated. Among all the tested samples, the xanthotoxin revealed the most potent cytotoxic activity with an IC50 of 6.9 μg/ ml.<br/> |
| 546 ## - LANGUAGE NOTE |
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| Language note |
Text in English, abstracts in English and Arabic. |
| 650 #4 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Ammi majus L. |
| 650 #4 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Aspergillus amstelodami, |
| 650 #4 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Drought stress |
| 650 #4 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
Ethephon |
| 650 #4 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
antioxidant |
| 650 #4 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
antimicrobial |
| 650 #4 - SUBJECT ADDED ENTRY--TOPICAL TERM |
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| Topical term or geographic name as entry element |
antibiofilm and cytotoxic activity |
| 856 40 - ELECTRONIC LOCATION AND ACCESS |
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| Materials specified |
DSpace electronic resource |
| Uniform Resource Identifier |
http://repository.fue.edu.eg/xmlui/handle/123456789/5785 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) |
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| Item type |
Thesis |