Laboratory manual of microbiology / (Record no. 5655)
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| 000 -LEADER | |
|---|---|
| fixed length control field | 06088nam a22002537i 4500 |
| 005 - DATE AND TIME OF LATEST TRANSACTION | |
| control field | 20201025105502.0 |
| 008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION | |
| fixed length control field | 120220s2011 ii a | |||| 00| 0 eng d |
| 020 ## - INTERNATIONAL STANDARD BOOK NUMBER | |
| International Standard Book Number | 9788172337063 |
| 040 ## - CATALOGING SOURCE | |
| Original cataloging agency | EG-NcFUE |
| Description conventions | rda |
| 082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER | |
| Edition number | 21 |
| Classification number | 616.9041 |
| Item number | K.V.L |
| 100 1# - MAIN ENTRY--PERSONAL NAME | |
| Personal name | Kumar, Vivek |
| 9 (RLIN) | 24817 |
| Relator term | author. |
| 245 10 - TITLE STATEMENT | |
| Title | Laboratory manual of microbiology / |
| Statement of responsibility, etc | Vivek Kumar. |
| 264 #1 - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) | |
| Place of publication, distribution, etc | New Delhi : |
| Name of publisher, distributor, etc | Scientific publishers, |
| Date of publication, distribution, etc | 2011 |
| 300 ## - PHYSICAL DESCRIPTION | |
| Extent | 214 pages : |
| Other physical details | illustrations ; |
| Dimensions | 24 cm |
| 336 ## - CONTENT TYPE | |
| Source | rdacontent |
| Content type term | text |
| 337 ## - MEDIA TYPE | |
| Source | rdamedia |
| Media type term | unmediated |
| 338 ## - CARRIER TYPE | |
| Source | rdacarrier |
| Carrier type term | volume |
| 504 ## - BIBLIOGRAPHY, ETC. NOTE | |
| Bibliography, etc | Includes bibliographical references |
| 505 0# - FORMATTED CONTENTS NOTE | |
| Formatted contents note | Exercises<br/>1 Microbiology laboratory practices and safety rules<br/>I BASIC LABORATORY TECHNIQUES<br/>2. Media preparation<br/> 2.1. Sub culturing or picking off:<br/> 2.2. Broth cultures<br/>3. Sterilization and disinfection<br/>4. Inoculation and other aseptic procedures<br/> 4.1. Using a wire loop<br/> 4.2. Flaming procedure<br/> 4.3. Using a pipette<br/> 4.4. Flaming the neck of bottles and test tubes<br/>5. Inoculation using a pipette<br/>6. Pouring the media in Petri plate<br/>7. Use of spreader<br/>8. Spread plate technique<br/>II MICROSCOPY<br/>9. Use and care of microscope<br/>10. Dark field microscopy<br/> 10.1. Bright field microscopy<br/> 10.2. Resolving power of microscope<br/> 10.3. Phase contrast field microscopy<br/> 10.4. Fluorescence microscopy<br/> 10.5. Electron microscopy<br/>11. Ocular micrometery<br/>III STAINING AND MOTILITY TECHNIQUES<br/>12. Staining preparations<br/>13. Smear making<br/>14. Simple staining<br/>15. Gram staining of bacteria<br/>16. Capsule staining of bacteria<br/>17. Capsule staining by negative, indirect or background staining <br/>18. Acid fast (Ziehl-Neelsen) staining of bacteria<br/>19. Endospore staining of bacteria<br/>20. Dorner’s method of endospore staining<br/>21. Bacterial flagella staining<br/>22. Viability staining technique of bacteria<br/>23. Lactophenol cotton blue staining of fungi<br/>24. Staining of Vesicular Arbuscular Mycorrhiza (VAM)<br/>25. Staining of actinomycetes culture<br/>26. Hanging drop slide for bacterial motility<br/>27. Motility Determination (Tube method)<br/>28. Slide technique for protozoa motility<br/>IV BIOCHEMICAL ACTIVITIES OF MICROBES<br/>29. Urease activity of bacteria<br/>30. Nitrate reduction test<br/>31. Catalase test<br/>32. Lipid hydrolysis test<br/>33. Oxidase test<br/>34. DNase production test<br/>35. Starch hydrolysis test or amylase production test<br/>36. Gelatin hydrolysis or production of gelatinase<br/>37. IMVIC tests<br/> 37.1. Indole Test<br/> 37.2. Methyl Red and Voges-Proskauer Tests<br/> 37.3. Citrate Test<br/>38. Hydrogen sulfide production and motility<br/>39. TSI (Triple Sugar Iron) and KIA (Kligler's Iron Agar)<br/>40. Casein hydrolysis<br/>41. Estimation of phytohormone IAA production from bacteria<br/>42. Estimation of ammonia production by bacteria<br/>43. Solubilization of mineral phosphate by microbes<br/>V ENVIRONMENTAL MICROBIOLOGY<br/>44. Multiple tube fermentation tests<br/> 44.1. Presumptive coliform test<br/> 44.2. Confirmed coliform test<br/> 44.3. Completed coliform test<br/>45. Counting of Ascaris eggs in raw or treated sewage water<br/>46. Determination of dissolved oxygen (DO) of water<br/>47. Determination of biological oxygen demand (BOD) of water<br/>48. Determination of chemical oxygen demand (COD) of water<br/>49. Detection of coliforms in water using membrane filtration technique<br/>VI MICROBIOLOGAL CULTURE TECHNIQUES<br/>50. Single Colony Isolation<br/>51. Viable plate count or Pour plate or serial dilution agar technique<br/>52. Counting of soil microorganisms<br/>53. Isolation of Rhizobium from root nodules<br/>54. Testing of nodulation ability by Rhizobia<br/>55. Isolation of Azotobacter from soil<br/>56. Carrier material for biofertilizers<br/>57. Seed treatment with biofertilizers<br/>58. Selection of bio-control agent<br/>59. Isolation of pure culture and their preservation<br/>60. Cultivation of anaerobic bacteria<br/>VII GROWTH OF LACTOSE FERMENTING AND NON- FERMENTING MICROBES<br/>61. Growth of bacteria using selective and differential media<br/>62. EMB (Eosine methylene blue) agar for E. coli<br/>63. MacConkey agar for enterobacterieaceae<br/>64. Salmonella-Shigella (SS) agar for Salmonella and Shigella<br/>65. XLD (Xylose Lysine Deoxycholate) agar for enterobacteriaceae<br/>VIII MEDICAL MICROBIOLOGY<br/>66. Micro-flora of upper respiratory tract<br/>67. Enumeration of urine bacteria using pour plate technique<br/>68. Use of urine dip slide for bacteuria examination<br/>69. Counting of bactiurea using calibrated loop method<br/>70. Widal test: The tube agglutination test<br/>71. Slide agglutination test for rapid diagnosis of pathogen<br/>72. The Ouchterlony technique (double immuno-diffusion method)<br/>73. Determination of Rh factor<br/>IX ENVIRONMENTAL EFFECT ON BACTERIAL GROWTH<br/>74. Effect of pH on bacterial growth<br/>75. Effect of oxygen on the growth of microorganisms<br/>76. Effect of temperature on growth of microorganisms<br/>77. Effect of osmotic pressure on growth of microorganisms<br/>78. Effect of radiation (Ultraviolet light) on growth of microorganism<br/>X APPLICATION OF MICROBIOLOGY<br/>79. Determination of viral plaque assay<br/>80. The one step bacteriophage growth curve<br/>81. Kirby-Bauer disk diffusion susceptibility test<br/>82. Determination of phenol coefficient of a chemical or effect of disinfectant on bacteria<br/>83. Determination of bacterial growth curve<br/>84. Demonstration of Koch's postulates<br/>85. Identifying Gram-negative enteric bacteria using API 20E system<br/>86. Identifying Gram-negative, oxidase negative enteric bacteria using Enterotube II system<br/>XI MICROBIOLOGY OF MILK<br/>87. Examination of milk quality by methylene blue reduction test<br/>88. Counting of number of bacteria in milk using Breed method <br/> APPENDICES<br/>A Preparation of desired alcohol concentration<br/>B Measurement units, conversion, factors and solution concentrations<br/>C Standard symbols and factors<br/>D Concentration normality<br/>E Symbols, atomic number and atomic weight of elements<br/>F Scientific notation<br/>G. Different culture media compositions<br/>H Reagents<br/>I Glossary<br/> REFERENCES |
| 650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM | |
| Topical term or geographic name as entry element | Microbiology |
| General subdivision | Laboratory manuals. |
| 9 (RLIN) | 24818 |
| 942 ## - ADDED ENTRY ELEMENTS (KOHA) | |
| Koha item type | Books |
| Source of classification or shelving scheme | Dewey Decimal Classification |
| Lost status | Source of classification or shelving scheme | Damaged status | Not for loan | Collection code | Home library | Current library | Shelving location | Date acquired | Source of acquisition | Cost, normal purchase price | Inventory number | Total Checkouts | Full call number | Barcode | Date last seen | Price effective from | Koha item type |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Dewey Decimal Classification | Pharmacy ( Microbiology ) | Main library | Main library | A11 | 20/02/2012 | Osiris Bookshop | 301.00 | PU | 616.9041 K.V.L | 00005630 | 19/02/2025 | 20/02/2012 | Books |