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Practical HPLC method development / Lloyd R. Snyder, Joseph J. Kirkland, Joseph L. Glajch.

By: Contributor(s): Material type: TextTextPublisher: New York : Wiley, c1997Copyright date: c1997Edition: second editionDescription: xxvi, 765 pages. : illustrations. ; 25 cmContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
ISBN:
  • 9780471007036
  • 9788126528530
  • 047100703X
Subject(s): DDC classification:
  • 543.0894 22 S.L.P
LOC classification:
  • QP519.9.H53 S69 1997
Online resources:
Contents:
Getting Started.; Basics of Separation.; Detection Sensitivity and Selectivity.; Sample Preparation.; The Column.; Non--Ionic Samples: Reversed-- and Normal--Phase HPLC.; Ionic Samples: Reversed--Phase, Ion--Pair, and Ion--Exchange HPLC.; Gradient Elution.; Systematic Approach to the Reversed--Phase Separation of Regular Samples.; Computer--Assisted Method Development.; Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds.; Chiral Separations.; Preparative HPLC Separation.; Quantitation (Including Trace Analysis).; Completing the Method: Validation and Transfer.; Appendices.; Index.
Summary: This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC.
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"A Wiley-Interscience publication."

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Includes bibliographical references and index.

Getting Started.; Basics of Separation.; Detection Sensitivity and Selectivity.; Sample Preparation.; The Column.; Non--Ionic Samples: Reversed-- and Normal--Phase HPLC.; Ionic Samples: Reversed--Phase, Ion--Pair, and Ion--Exchange HPLC.; Gradient Elution.; Systematic Approach to the Reversed--Phase Separation of Regular Samples.; Computer--Assisted Method Development.; Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds.; Chiral Separations.; Preparative HPLC Separation.; Quantitation (Including Trace Analysis).; Completing the Method: Validation and Transfer.; Appendices.; Index.

This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC.

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