Advances in molecular techniques / editors, Rakesh S. Sengar, Amit Kumar, Reshu Chaudhary, Ashu Singh.

Includes bibliographical references and index.

Cover; Half Title; Title Page; Copyright Page; Dedication; Contents; Foreword; Preface; Authors; 1. DNA Manipulative and Ingratiative Techniques; Isolation of Total Genomic DNA; Isolation of Plant DNA Using the CTAB Method; Introduction; Experimental Outline; Materials Required; Pre-Lab Preparation; Isolation of Plant DNA Using the SDS Method; Introduction; Materials Required; Method; Precautions; To Isolate Plasmid DNA from Bacteria; Introduction; Materials Required; Method; Precautions; Isolation of Chloroplasts; Introduction; Experimental Outline; Materials Required; Pre-Lab Preparation. MethodResults; Precautions; Isolation of Chloroplast DNA; Introduction; Experimental Outline; Materials Required; Pre-Lab Preparation; Method; Results; Precautions; Isolation of Yeast Genomic DNA; Introduction; Materials Required; Method; Isolation of Bacterial Genomic DNA; Introduction; Materials Required; Method; Large-Scale Isolation of Cosmid DNA; Isolation; Purification; Miniprep Double-Stranded DNA Isolation; Principle; Isolation; Standard Alkaline Lysis Purification; Diatomaceous Earth-Base Purification; Plasmid Miniprep by Birnboim Method; Materials Required. Plasmid Miniprep by Boiling MethodMaterials Required; Method; Isolation of Lambda (.) DNA; Materials Required; Method; Quick Method for isolation of Lambda (.) DNA; Materials Required; Single-Stranded M13 DNA Isolation Using Phenol; Method; Biomek-Automated Modified-Eperon Isolation Procedure for Single-Stranded M13 DNA; Method; Isolation of Aspergillus DNA; Materials Required; Method; Isolation of Aspergillus DNA by the Qiagen Method; Materials Required; Method; Purification of Genomic DNA; Introduction; Principle; Materials Required; Method; Materials Required. Isolation of RNA from Plant TissuesIntroduction; Extraction of Total RNA; Materials Required; Method; Precautions; Extraction of Polysomal RNA of Nuclear Sequences; Materials Required; Chemicals; Glassware and Other; Equipment; Isolation of Total RNA form Bacterial Cells; Introduction; Materials Required; Method; Quantitative Determination of DNA and RNA by a Spectrophotometric Method; Introduction; Materials Required; Agarose Gel Electrophoresis; Introduction; Preparation of Agarose Gel; Electrophoresis of DNA Fragments; Visualization of DNA Fragments; Materials Required; Chemicals. Glassware and OtherEquipment; Electrophoresis of DNA: Linear, Circular, and Super Coiled; Introduction; Experimental Outline; Materials Required; Method; Precautions; Elution of Plant DNA Fragment from Agarose Gel; Introduction; Materials Required; Method; Precautions; Hybridization and Autoradiography of DNA; Introduction; Experimental Outline; Materials Required; Method; Hybridization; Autoradiography; Precautions; PCR in Outline; Introduction; Choice Between Cloning and PCR; Experimental Outline; Materials Required; Method; Precautions.