Fundamentals of microfluidics and lab on a chip for biological analysis and discovery / Paul C.H. Li.

Includes bibliographical references and index.

IntroductionMicromachining MethodsMicromachining of SiliconMicromachining of GlassMicromachining of Fused Quartz (or Fused Silica)Micromachining of Polymeric ChipsMetal PatterningWorld-to-Chip InterfaceProblem SetsMicrofluidic FlowLiquid Pumping MethodsMicrofluidic Flow ControlProblem SetsSample IntroductionElectrokinetic InjectionHydrodynamic InjectionOther Sample Injection MethodsProblem SetsSample PreconcentrationSample StackingExtractionPorous MembraneOther Preconcentration MethodsProblem SetsSeparationGas Chromatography (GC)Capillary Electrophoresis (CE)Chromatographic SeparationsCoupled SeparationsProblem SetsDetection MethodsOptical Detection MethodsElectrochemical (EC) DetectionMass Spectrometry (MS)Other Detection MethodsProblem SetsApplications to Cellular/Particle AnalysisRetention of Cells and ParticlesStudies of Cells in a FlowOther Cell OperationsProblem SetsApplications to Nucleic Acids AnalysisNucleic Acids Extraction and PurificationNucleic Acids AmplificationDNA HybridizationOther Nucleic Acid ApplicationsProblem SetsApplications to Protein AnalysisImmunoassayProtein SeparationEnzymatic AssaysProblem SetsReferencesGlossaryAppendixIndex

Lab-on-a-chip technology permits us to make many important discoveries that can only be observed at the microscale or the nanoscale. This book focuses on the key aspects of microfluidic lab-on-a-chip technologies to offer an overview of the science, its limitations, breakthroughs made over the years, and the emerging advances.