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Benson's microbiological applications : laboratory manual in general microbiology, complete version / Alfred Brown.

By: Contributor(s): Material type: TextTextPublication details: Boston [u.a.] : McGraw-Hill, c 2007.Edition: 10th edDescription: XIV, 543 S. : Ill., graph. DarstContent type:
  • text
Media type:
  • unmediated
Carrier type:
  • volume
ISBN:
  • 0071254161 (pbk.)
  • 9780071254168 (pbk.)
  • 0072992727 (hbk.)
  • 9780072992724 (hbk.)
Subject(s): DDC classification:
  • 579.078 B.A.B 22
LOC classification:
  • QR63
Online resources:
Contents:
Part 1. Microscopy -- Brightfield Microscopy -- Darkfield Microscopy -- Phase-Contrast Microscopy -- Fluorescence Microscopy -- Microscopic Measurements -- Part 2. Survey of Microorganisms -- Protozoans, Algae, and Cyanobacteria -- Ubiquity of Bacteria -- The Fungi: Yeasts and Molds -- Part 3. Manipulation of Microorganisms -- Aseptic Technique -- Pure Culture Techniques -- Part 4. Staining and Observation of Microorganisms -- Smear Preparation -- Simple Staining -- Negative Staining -- Capsular Staining -- Gram Staining -- Spore Staining: Two Methods -- Acid-Fast Staining: Ziehl-Neelsen Method -- Motility Determination -- Part 5. Culture Methods -- Culture Media Preparation -- Preparation of Stock Cultures -- Cultivation of Anaerobes -- Enumeration of Bacteria: The Standard Plate Count -- Slime Mold Culture -- Slide Culture: Molds -- Part 6. Bacterial Viruses -- Determination of a Bacteriophage Titer -- Burst Size Determination: A One-Step Growth Curve -- Isolation of Phage from Flies -- Phage Typing -- Part 7. Environmental Influences and Control of Microbial Growth -- Temperature: Effects on Growth -- Temperature: Lethal Effects -- pH and Microbial Growth -- Water Activity and Osmotic Pressure -- Ultraviolet Light: Lethal Effects -- The Effects of Lysozyme on Bacterial Cells -- Evaluation of Alcohol: Its Effectiveness as an Antiseptic -- Antimicrobic Sensitivity Testing: The Kirby-Bauer Method -- Evaluation of Antiseptics: The Filter Paper Disk Method -- Effectiveness of Hand Scrubbing --
Summary: The classic resource for undergraduate microbiology laboratory courses just keeps getting better. The self-contained clearly illustrated exercises and full-color format make Microbiological Applications: Laboratory Manual in General Microbiology the ideal lab manual. Appropriate for either a majors or non-majors lab course this manual assumes no prior organic chemistry course has been taken. Features • Content has been updated throughout especially in Part 8: Identification of Unknown Bacteria and in Part 13: Medical Microbiology and Immunology to include the latest research. Also updated was Exercise 78: A Synthetic Epidemic in keeping with lab safety requirements. • Benson s Complete Version (78 experiments) provides the largest variety of laboratory exercises to accommodate all laboratory curricula--from a medical emphasis to an environmental emphasis and everything between. (A Short Version with 60 experiments is also available.) • Full-color examples of lab test results appear in the lab exercises so students have an immediate reference against which they can compare their results. • Because you may prefer or require a different way to stage a lab exercise this lab manual includes suggestions for alternative approaches and procedures in the appendixes and in the Instructor s Manual. • The Instructor s Manual provides a materials list and guidelines for conducting all the experiments as well as answers to the lab exercises. It can be found at www.mhhe.com/labcentral • Customize this book through Primis Online! This title is part of the Primis Online Database: www.mhhe.com/primis/online. You can customize this book to meet your exact needs and mix and match with other items on Primis Online--allowing you maximum choice and flexibility. Instructors can also choose between two delivery formats: custom printed books (in black and white) or custom eBooks (in color).
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BIO257OLD.

Previous ed.: 2005.

Part 1. Microscopy --
Brightfield Microscopy --
Darkfield Microscopy --
Phase-Contrast Microscopy --
Fluorescence Microscopy --
Microscopic Measurements --
Part 2. Survey of Microorganisms --
Protozoans, Algae, and Cyanobacteria --
Ubiquity of Bacteria --
The Fungi: Yeasts and Molds --
Part 3. Manipulation of Microorganisms --
Aseptic Technique --
Pure Culture Techniques --
Part 4. Staining and Observation of Microorganisms --
Smear Preparation --
Simple Staining --
Negative Staining --
Capsular Staining --
Gram Staining --
Spore Staining: Two Methods --
Acid-Fast Staining: Ziehl-Neelsen Method --
Motility Determination --
Part 5. Culture Methods --
Culture Media Preparation --
Preparation of Stock Cultures --
Cultivation of Anaerobes --
Enumeration of Bacteria: The Standard Plate Count --
Slime Mold Culture --
Slide Culture: Molds --
Part 6. Bacterial Viruses --
Determination of a Bacteriophage Titer --
Burst Size Determination: A One-Step Growth Curve --
Isolation of Phage from Flies --
Phage Typing --
Part 7. Environmental Influences and Control of Microbial Growth --
Temperature: Effects on Growth --
Temperature: Lethal Effects --
pH and Microbial Growth --
Water Activity and Osmotic Pressure --
Ultraviolet Light: Lethal Effects --
The Effects of Lysozyme on Bacterial Cells --
Evaluation of Alcohol: Its Effectiveness as an Antiseptic --
Antimicrobic Sensitivity Testing: The Kirby-Bauer Method --
Evaluation of Antiseptics: The Filter Paper Disk Method --
Effectiveness of Hand Scrubbing --

The classic resource for undergraduate microbiology laboratory courses just keeps getting better. The self-contained clearly illustrated exercises and full-color format make Microbiological Applications: Laboratory Manual in General Microbiology the ideal lab manual. Appropriate for either a majors or non-majors lab course this manual assumes no prior organic chemistry course has been taken.
Features
• Content has been updated throughout especially in Part 8: Identification of Unknown Bacteria and in Part 13: Medical Microbiology and Immunology to include the latest research. Also updated was Exercise 78: A Synthetic Epidemic in keeping with lab safety requirements.
• Benson s Complete Version (78 experiments) provides the largest variety of laboratory exercises to accommodate all laboratory curricula--from a medical emphasis to an environmental emphasis and everything between. (A Short Version with 60 experiments is also available.)
• Full-color examples of lab test results appear in the lab exercises so students have an immediate reference against which they can compare their results.
• Because you may prefer or require a different way to stage a lab exercise this lab manual includes suggestions for alternative approaches and procedures in the appendixes and in the Instructor s Manual.
• The Instructor s Manual provides a materials list and guidelines for conducting all the experiments as well as answers to the lab exercises. It can be found at www.mhhe.com/labcentral
• Customize this book through Primis Online! This title is part of the Primis Online Database: www.mhhe.com/primis/online. You can customize this book to meet your exact needs and mix and match with other items on Primis Online--allowing you maximum choice and flexibility. Instructors can also choose between two delivery formats: custom printed books (in black and white) or custom eBooks (in color).

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