TY  - MANSCPT
AU  - Elhefnawy,Esraa Akram Helmi
TI  - Implication of JAK/STAT Signaling Pathway on Acetic Acid-Induced Ulcerative Colitis in Rats
U1  - 615.1 22
PY  - 2023///
KW  - Pharmacology
KW  - Toxicology
KW  - Ulcerative colitis
KW  - Genistein
KW  - Sulfasalazine
KW  - Acetic acid
N1  - By Esraa Akram Helmi Elhefnawy, (Bachelor’s Degree of Pharmacy, Faculty of Pharmacy, Future University in Egypt (2017), Teaching Assistant, Pharmacology, Toxicology & Biochemistry Department, Faculty of Pharmacy, Future University in Egypt, Under the Supervision of Dr. Hala Fahmy Zaki, Professor of Pharmacology and Toxicology, Faculty of Pharmacy, Cairo University, Dr. Nabila El-Maraghy, Professor of Pharmacology and Toxicology & Vice Dean of Community Services & Environmental Development, Faculty of Pharmacy, Future University in Egypt, Dr. Enas Abd El-Haleim, Assistant Professor of Pharmacology and Toxicology, Faculty of Pharmacy, Cairo University; Thesis (M.Sc.)-Cairo University, Faculty of Pharmacy, Department of Pharmacology and Toxicology, 2023; Includes bibliographical references
N2  - Ulcerative Colitis (UC) is a chronic idiopathic inflammatory bowel disease in which the colon’s lining becomes inflamed. Exploring herbal remedies that can recover mucosal damage is becoming popular in UC. The study aims to investigate the probable colo-protective effect of a natural isoflavone, genistein (GEN), and/or sulfasalazine (SZ), against acetic acid (AA)-induced UC in rats, in addition to exploring the possible underlying mechanisms. UC was induced by the intrarectal instillation of 1-2 ml of 5% AA for 24 hours. AA-induced UC rats were allocated into the disease group and three treated groups, with SZ (100 mg/kg), GEN (100 mg/kg), and their combination for 14 days, besides the control groups. The anti-colitic efficacy of GEN and/or SZ was evidenced by hindering the AA-induced weight loss, colon edema, and macroscopic scores, besides reduced disease activity index and colon weight/length ratio. Furthermore, treatments attenuated the colon histopathological injury scores, increased the number of goblet cells, and decreased fibrosis. Both treatments reduced the up-regulation of INF-γ/JAK1/STAT1 and INF-γ /TLR-4/ NF-κB signaling pathways and modulated the IRF-1/iNOS/NO and IL-6/JAK2/STAT3/COX-2 pathways and consequently, reduced the levels of TNF-α and IL-1β. Moreover, both treatments diminished oxidative stress, by reducing the myeloperoxidase (MPO) level and elevating the superoxide dismutase (SOD) activity, and hindered apoptosis; proved by the decreased immunohistochemical expression of caspase-3. The current findings offer novel insights into the protective effects of GEN and suggest a superior benefit of combining GEN with SZ, over either drug alone, in the UC management.
ER  -