TY  - BOOK
AU  - Norrell,Stephen A.
AU  - Messley,Karen E.
TI  - Microbiology laboratory manual : : principles and applications 
SN  - 0130100293
U1  - 616.01 21
PY  - 2003///]
CY  - Upper Saddle River, N.J.
PB  - Prentice-Hall
KW  - Microbiology
KW  - Laboratory manuals
KW  - Microbiological Techniques
N1  - I. MICROSCOPIC TECHNIQUES. 1. Introduction to Microscopy. 2. Preparation of Smears and Simple Stains. 3. Wet Mounts and Hanging Drops. 4. The Negative Stain. 5. Differential Staining Procedures: Gram Stain, Acid-Fast Stain. 6. Additional Differential Staining Procedures: Metachromatic-Granules, Capsule, Endospore and Flagella Stains. II. MICROBIAL DIVERSITY. 7. Ubiquity of Microorganisms. 8. Survey of Microorganisms. III. MICROBIAL CULTIVATION TECHNIQUES. 9. Isolation Techniques: Streak Plate. 10. Isolation Techniques: Pour Plates. 11. Oxygen Requirements of Microorganisms. 12. Physical Growth Requirements. 13. Selective and Differential Media. 14. Physiological Characteristics of Bacteria: Carbohydrate Metabolism. 15. Physiologic Characteristics of Bacteria: Reactions of Nitrogen Metabolism. 16. Physiologic Characteristics of Bacteria: Miscellaneous Reactions. 17. Multiple Test Media. IV. IDENTIFICATION TECHNIQUES. 18. Development and Use of Diagnostic Keys. 19. Use of Diagnostic Keys: Bacterial Unknowns. 20. Rapid Diagnostic Tests: Profile Analysis. V. MICROBIAL GROWTH. 21. Microbial Population Counts: Microscopic Methods. 22. Microbial Population Counts: Viable Cell Counts. VI. MICROBIAL CONTROL. 23. Physical Control Methods. 24. UV Light as an Antimicrobial Agent. 25. Assay of Antimicrobial Agents: Disc-Diffusion Methods. 26. Assay of Antimicrobial Agents: Use-Dilution Methods. 27. Antibiotic Sensitivity Testing: The Kirby-Bauer Procedure. 28. Detection of Mutant Strains of Bacteria. VII. CLINICAL MICROBIOLOGY. 29. Epidemiology. 30. Serological Reactions. 31. The Collection and Transport of Clinical Specimen. 32. Throat Cultures. 33. Urinary Tract Cultures. 34. Gastrointestinal Tract Cultures. 35. Lactobacillus Activity. VIII. VIROLOGY. 36. Viral Population Counts: Plaque Counting and Plaque Morphology. IX. APPLIED MICROBIOLOGY. 37. Food Microbiology. 38. Water Analysis: Standard Methods. 39. Water Analysis: Membrane Filter Techniques. Appendix A: Writing a Lab Report. Appendix B: Cultures Used. Appendix C: Media, Stains, Reagents, and Supplies

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