TY - BOOK AU - Arora,Dilip K. AU - Das,Surajit AU - Sukumar,Mesapogu TI - Analyzing microbes: manual of molecular biology techniques T2 - Springer protocols handbooks SN - 9783642344091 AV - QH506 .A52 2013 U1 - 572.8 22 PY - 2013/// CY - Heidelberg, New York PB - Springer KW - Molecular biology KW - Molecular Biology KW - methods KW - Microbiological Phenomena N1 - Includes bibliographical references and index; Microbial DNA extraction, purification, and quantitation; Fluorescent-based detection, quantitation, and expression of viral gene by qRT-PCR; Restriction enzymes and their role in microbiology; Genetic fingerprinting techniques for molecular characterisation of microbes; Agarose gel electrophoresis and polyacrylamide gel electrophoresis : methods and principles; Molecular identification of microbes : I. Macrophomina phaseolina; Molecular identification of microbes : II. Bacillus; Molecular identification of microbes : III. Pseudomonas; Molecular identification of microbes : IV. Vibrio; Molecular identification of microbes : V. methylotrophs; Preservation and maintenance of microbial cultures; Microbes from extreme environment : molecular identification procedures; ELISA-based identification and detection of microbes; Analysis of microbial diversity and construction of metagenomic library; Bioinformatics tools for interpretation of data used in molecular identification; Molecular phylogenetics of microbes; Microarray technology : basic concept, protocols, and applications; Microarray analysis of different functional genes of microorganisms; DNA cloning and sequencing; Biological sequence analysis : algorithms and statistical methods; Appendix 1; Biosafety, GLP, and biosecurity --; Appendix 2; Address for instruments and chemicals suppliers UR - http://repository.fue.edu.eg/xmlui/handle/123456789/2776 ER -