TY  - BOOK
AU  - Bina,Minou
TI  - Gene regulation: methods and protocols 
T2  - Methods in molecular biology
SN  - 9781627032834 (alk. paper)
AV  - QH450 .G4629 2013
U1  - 572.865 22
PY  - 2013///
CY  - New York
PB  - Humana Press, Springer
KW  - Genetic regulation
KW  - Laboratory manuals
KW  - Gene Expression Regulation
KW  - Laboratory Manuals
N1  - Includes bibliographical references and index; Gene regulation; Minou Bina --; Isolation of nuclei for use in genome-wide DNase hypersensitivity assays to probe chromatin structure; Guoyu Ling and David J. Waxman --; DNase I digestion of isolated nulcei for genome-wide mapping of DNase hypersensitivity sites in chromatin; Guoyu Ling and David J. Waxman --; Isolation and analysis of DNA derived from nucleosome-free regions; Matthew Murtha ... [et al.] --; Acquisition of high quality DNA for massive parallel sequencing by in vivo chromatin immunoprecipitation; M. van den Boogaard ... [et al.] --; Luciferase assay to study the activity of a cloned promoter DNA fragment; Nina Solberg and Stefan Krauss --; Promoter deletion analysis using a dual-luciferase reporter system; Yong Zhong Xu ... [et al.] --; Application of mRNA display for in vitro selection of DNA-binding transcription factor complexes; Seiji Tateyama and Hiroshi Yanagawa --; Isolation of intracellular protein - DNA complexes using HaloCHIP an antibody-free alternative to chromatin immunoprecipitation; Danette L. Daniels and Marjeta Urh --; Modified yeast one-hybrid system for genome-wide identification of transcription factor binding sites; Kazuyuki Yanai --; Identifying specific protein-DNA interactions using SILAC-based quantitative proteomics; Cornelia G. Spruijt, H. Irem Baymaz, and Michiel Vermeulen --; Electrophoretic mobility-shift and super-shift assays for studies and characterization of protein-DNA complexes; Elsie I. Pares-Matos --; Combination of native and denaturing PAGE for the detection of protein binding regions in long fragments of genomic DNA; Kristel Kaer and Mart Speek --; Quantitative nanoproteomics approach for protein complex (QNanoPX) using gold nanoparticle-based DNA probe; Shu-Hui Chen and Mei-Yin Lin --; Chromatin assembly and in vitro transcription analyses for evaluation of individual protein activities in multicomponent transcriptional complexes; Takayuki Furumatsu and Hiroshi Asahara --; Using FRET to monitor protein-induced DNA bending : the TBP-TATA complex as a model system; Rebecca H. Blair, James A. Goodrich, and Jennifer F. Kugel --; Promoter independent abortive transcription assays unravel functional interactions between TFIIB and RNA polymerase; Simone C. Wiesler, Finn Werner, and Robert O.J. Weinzierl --; Fluorescence cross-correlation spectroscopy (FCCS) to observe dimerization of transcription factors in living cells; Hisayo Sadamoto and Hideki Muto ---; Nuclear recruitment assay as a tool to validate transcription factor interactions in mammalian cells; C.J.J. Boogerd, V.M. Christoffels, and P. Barnett --; Preparation of cell lines for single-cell analysis of transcriptional activation dynamics; Ilona U. Rafalska-Metcalf and Susan M. Janicki --; Peptide microarrays for profiling of serine/threonine kinase activity of recombinant kinases and lysates of cells and tissue samples; Riet Hilhorst ... [et al.] --; Immunoaffinity purification of protein complexes from mammalian cells; Chieri Tomomori-Sato ... [et al.] --; Simple and efficient identification of chromatin modifying complexes and characterization of complex composition; Jeong-Heon Lee and David Skalnik --; Heavy methyl-SILAC labeling coupled with liquid chromatography and high-resolution mass spectrometry to study the dynamics of site-specific histone methylation; Xing-Jun Cao, Barry M. Zee, and Benjamin A. Garcia --; Analysis of p300 occupancy at the early stage of stem cell differentiation by chromatin immunoprecipitation; Melanie Le May and Qiao Li --; Mammalian two-hybrid assays for studies of interaction of p300 with transcription factors; Daniela B. Mendonca, Gustavo Mendonca, and Lyndon F. Cooper --; Fluorescence anisotropy microplate assay to investigate the interaction of full-length steroid receptor coactivator-1a with steroid receptors; Chen Zhang, Steven K. Nordeen, and David J. Shapiro --; Use of histone deacetylase inhibitors to examine the roles of bromodomain and histone acetylation in p300-dependent gene expression; Jihong Chen and Qiao Li --; Histone deacetylase inhibitor valproic acid as a small molecule inducer to direct the differentiation of pluripotent stem cells; Jihong Chen, Natascha Lacroix, and Qiao Li --; Sedimentation and immunoprecipitation assays for analyzing complexes that repress transcription; Ping Lu ... [et al.] --; Methods for studies of protein interactions with different DNA methyltransferases; Jianchang Yang
UR  - http://repository.fue.edu.eg/xmlui/handle/123456789/2855
ER  -