Design and evaluation of statin loaded biodegradable delivery systems to promote tissue regeneration / Asst. Lec. Amr Maged Ibrahim, (Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Future University in Egypt), supervisions, Prof. Mahmoud M.Ghrab, Ph.D., (Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Egypt.), Prof. Azza Ahmed Mahmoud, Ph.D., (Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Future University in Egypt), Prof. Salwa Mohamed Salah El Din, Ph.D., (Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Egypt).

By:

ibrahim, Amr Maged [author.]

Material type: Text

TextLanguage: English Summary language: Arabic Publisher: 2020Description: 187 pages, 7 pages : illustrations (some color) ; 24 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Other title:

تصميم و تقييم نظم محملة بعقار ستاتين قابلة للتحل البيولوجي لتحفيز تجديد الأنسجة [Added title page title]

Subject(s):

DDC classification:

615.19 I.A.D 22

Online resources:

Dspace Electronic resource

Dissertation note: Thesis (Ph.D.)- Cairo university, Faculty of pharmacy, Department of Pharmaceutics and industrial Pharmacy, 2020. Abstract: In this study, we explored the role of rosuvastatin calcium in skin regeneration as statins play important role in the field of tissue engineering. Chitosan hydrochloride or sodium alginate were crosslinked with different weight ratios of collagen, β-glycerolphosphate and carboxymethyl cellulose to produce scaffolds by lyophilization technique. Subsequently, the fabricated scaffolds were examined for their morphology, water retention capacity, friability and in-vitro drug release studies as well as in-vivo study. The results revealed porous 3-D structure with maximum water retention-values ranging between 179 and 2993%. Scaffolds containing carboxymethyl cellulose revealed highest water retention-values. In-vitro drug release results showed gradual drug release for 60 h with mean dissolution time-values (MDT) between 2 and 21 h. Combination of chitosan, collagen, carboxymethyl cellulose in weight ratio of 40:30:30, respectively achieved gradual disintegration of the scaffold in a simulating medium to an open wound after 4 days. This selected scaffold loaded with rosuvastatin revealed increase proliferation of human dermal fibroblasts compared to placebo scaffold. After 30 days of implantation of selected medicated scaffold loaded with/without mesenchymal stem cells and placebo scaffolds to induced wounds in Albino rats, enhanced skin regeneration and absence of scar formation for drug loaded scaffolds was observed. The histopathological study showed the advantage of stem cells loaded scaffolds through the normal redistribution of collagen in the epidermal layer. In conclusion, rosuvastatin calcium and stem cells loaded in tested scaffolds proved their potential effect in enhancing skin healing and regeneration.

Average rating: 0.0 (0 votes)

Holdings
Item type	Current library	Collection	Call number	Status	Date due	Barcode
Thesis	Main library C5 PHD	Pharmacy ( Pharmaceutical Technology )	615.19 I.A.D (Browse shelf(Opens below))	Not for loan		00016478

Supervisions, Prof. Mahmoud M.Ghrab, Ph.D., (Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Egypt.), Prof. Azza Ahmed Mahmoud, Ph.D., (Department of Pharmaceutics and Pharmaceutical Technology, Faculty of Pharmaceutical Sciences and Pharmaceutical Industries, Future University in Egypt), Prof. Salwa Mohamed Salah El Din, Ph.D., (Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Egypt).

Thesis (Ph.D.)- Cairo university, Faculty of pharmacy, Department of Pharmaceutics and industrial Pharmacy, 2020.

Includes bibliographical references.

In this study, we explored the role of rosuvastatin calcium in skin regeneration as statins play important role in the field of tissue engineering. Chitosan hydrochloride or sodium alginate were crosslinked with different weight ratios of collagen, β-glycerolphosphate and carboxymethyl cellulose to produce scaffolds by lyophilization technique. Subsequently, the fabricated scaffolds were examined for their morphology, water retention capacity, friability and in-vitro drug release studies as well as in-vivo study. The results revealed porous 3-D structure with maximum water retention-values ranging between 179 and 2993%. Scaffolds containing carboxymethyl cellulose revealed highest water retention-values. In-vitro drug release results showed gradual drug release for 60 h with mean dissolution time-values (MDT) between 2 and 21 h. Combination of chitosan, collagen, carboxymethyl cellulose in weight ratio of 40:30:30, respectively achieved gradual disintegration of the scaffold in a simulating medium to an open wound after 4 days. This selected scaffold loaded with rosuvastatin revealed increase proliferation of human dermal fibroblasts compared to placebo scaffold. After 30 days of implantation of selected medicated scaffold loaded with/without mesenchymal stem cells and placebo scaffolds to induced wounds in Albino rats, enhanced skin regeneration and absence of scar formation for drug loaded scaffolds was observed. The histopathological study showed the advantage of stem cells loaded scaffolds through the normal redistribution of collagen in the epidermal layer. In conclusion, rosuvastatin calcium and stem cells loaded in tested scaffolds proved their potential effect in enhancing skin healing and regeneration.

Text in English, abstracts in English and Arabic.

There are no comments on this title.

to post a comment.