Impact of Some Long Noncoding RNAs Expression on the Occurrence and Development of Cerebral Stroke in Relation to Hypoxia Inducible Factor-1 in Egyptian patients / By Rana Kamal El-Din Ahmed Zayed Teaching assistant Biochemistry Section Faculty of Pharmacy Future University in Egypt ; Supervisors : Prof. Dr. Hebatallah A. M. Darwish Professor of Biochemistry Faculty of Pharmacy Future University in Egypt, Prof. Dr. Amira A. Shaheen Professor of Biochemistry Faculty of Pharmacy Cairo University.

By:

Zayed, Rana Kamal El-Din Ahmed [author.]

Contributor(s):

Material type: Text

TextLanguage: English Summary language: Arabic Producer: 2022Description: 122 pages : illustrations (some color) ; 24 cmContent type:

text

Media type:

unmediated

Carrier type:

volume

Other title:

أثر التعبير الجيني لبعض الأحماض النووية الريبوزية الطويلة غير المشفرة على حدوث وتطور مرض السكتة الدماغية وعلاقته بالعامل المحفز لنقص الأكسجين-1 في المرضى المصريين

Subject(s):

DDC classification:

23 572.88 Z.R.I

Dissertation note: Thesis (M.Sc.)-Future University in Egypt, Faculty of pharmacy, Department of Biochemistry Section, 2022. Abstract: Long noncoding RNAs (lncRNAs) have been recently recognized as key players of gene expression in cerebral pathogenesis. Thus, their potential use in stroke diagnosis, prognosis, and therapy is actively pursued. Due to the complexity of the disease, identifying stroke-specific lncRNAs remains a challenge. This study investigated the expression of lncRNAs HIF1A-AS2 and LINK-A, and their target gene hypoxia-inducible factor-1 (HIF-1) in Egyptian stroke patients. It also aimed to determine the molecular mechanism implicated in the disease. A total of 75 stroke patients were divided into three clinical subgroups, besides 25 healthy controls of age-matched and sex-matched. Remarkable upregulation of lncRNA HIF1A-AS2 and HIF1-α along with a downregulation of lncRNA LINK-A was noticed in all stroke groups relative to controls. Serum levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated-AKT (p-AKT), vascular endothelial growth factor (VEGF), and angiopoietin-1 (ANG-1) as well as their receptors, malondialdehyde (MDA), and total antioxidant capacity (TAC) were significantly increased, whereas brain-derived neurotrophic factor (BDNF) levels were significantly decreased particularly in hemorrhagic stroke versus ischemic groups. Eventually, these findings support the role of lncRNAs HIF1A-AS2 and LINK-A as well as HIF1-α in activation of angiogenesis, neovascularization, and better prognosis of stroke, especially the hemorrhagic type.

Tags from this library: No tags from this library for this title. Log in to add tags.

Average rating: 0.0 (0 votes)

Holdings
Item type	Current library	Collection	Call number	Copy number	Status	Date due	Barcode
Thesis	Main library C3 THESIES	Pharmacy ( Pharmaceutical chemistry )	572.88 Z.R.I (Browse shelf(Opens below))	c.1	Not for loan		00016646

Supervisors : Prof. Dr. Hebatallah A. M. Darwish Professor of Biochemistry Faculty of Pharmacy Future University in Egypt, Prof. Dr. Amira A. Shaheen Professor of Biochemistry Faculty of Pharmacy Cairo University.

Thesis (M.Sc.)-Future University in Egypt, Faculty of pharmacy, Department of Biochemistry Section, 2022.

Includes bibliographical references (100-122 p.)

Long noncoding RNAs (lncRNAs) have been recently recognized as key players of gene expression in cerebral pathogenesis. Thus, their potential use in stroke diagnosis, prognosis, and therapy is actively pursued. Due to the complexity of the disease, identifying stroke-specific lncRNAs remains a challenge. This study investigated the expression of lncRNAs HIF1A-AS2 and LINK-A, and their target gene hypoxia-inducible factor-1 (HIF-1) in Egyptian stroke patients. It also aimed to determine the molecular mechanism implicated in the disease. A total of 75 stroke patients were divided into three clinical subgroups, besides 25 healthy controls of age-matched and sex-matched. Remarkable upregulation of lncRNA HIF1A-AS2 and HIF1-α along with a downregulation of lncRNA LINK-A was noticed in all stroke groups relative to controls. Serum levels of phosphatidylinositol 3-kinase (PI3K), phosphorylated-AKT (p-AKT), vascular endothelial growth factor (VEGF), and angiopoietin-1 (ANG-1) as well as their receptors, malondialdehyde (MDA), and total antioxidant capacity (TAC) were significantly increased, whereas brain-derived neurotrophic factor (BDNF) levels were significantly decreased particularly in hemorrhagic stroke versus ischemic groups. Eventually, these findings support the role of lncRNAs HIF1A-AS2 and LINK-A as well as HIF1-α in activation of angiogenesis, neovascularization, and better prognosis of stroke, especially the hemorrhagic type.

There are no comments on this title.

to post a comment.