000			04180ntm a2200301 i 4500
999			_c13129 _d13129
005			20230110112541.0
008			150118s2022 ua a f bm 000 0 eng |
040			_aEG-EULC _cEG-EULC _erda
041	0		_aeng _bara
082	0	4	_a572 _bA.M.S _222
100	1		_aAli, Mohamed Salah Attia Radwan, _933980 _eauthor.
245	1	0	_aStudying the possible role of some long noncoding RNAs in multiple sclerosis / _cBy Mohamed Salah Attia Radwan Ali, Teaching assistant, Biochemistry Section, Faculty of Pharmacy, Future University in Egypt; Supervision of Prof. Dr. Maha M. El-Sawalhi, Professor of Biochemistry, Faculty of Pharmacy, Cairo University, Prof. Dr. Shohda A. El-Maraghy, Professor of Biochemistry, Faculty of Pharmacy, Cairo University, Ass. Prof. Dr. Hebatollah Atef Ewida, Associate Professor of Biochemistry, Faculty of Pharmacy, Future University in Egypt.
246	1	5	_aدراسة الدور المحتمل لبعض الأحماض النووية الريبوزية الطويلة غير المشفرة في التصلب المتعدد
264		1	_c2022.
300			_aviii, 138 pages, 4 pages : _billustrations ; _c22 cm
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
500			_aSupervision of Prof. Dr. Maha M. El-Sawalhi, Professor of Biochemistry, Faculty of Pharmacy, Cairo University, Prof. Dr. Shohda A. El-Maraghy, Professor of Biochemistry, Faculty of Pharmacy, Cairo University, Ass. Prof. Dr. Hebatollah Atef Ewida, Associate Professor of Biochemistry, Faculty of Pharmacy, Future University in Egypt
502			_aThesis (M.Sc.)-Future University in Egypt, Faculty of pharmacy, Department of Biochemistry, 2022.
504			_aIncludes bibliographical references.
520	3		_aMultiple sclerosis (MS) is the primary cause of non-traumatic neurological disability in young adults. Relapsing-remitting MS (RRMS) is the most prevalent MS subtype. Ample evidence indicated that long noncoding RNAs (lncRNAs) are crucial players in autoimmune and inflammatory disorders. Despite the rapidly increasing data concerning MS-related lncRNAs, the impact of others remains to be explored. This study investigated for the first time the expression profiles of lnc-EGFR, SNHG1, and lincRNA-Cox2 in Egyptian patients with RRMS during active relapses and in remission. Besides, the expression of FOXP3, a master transcription factor for T-regulatory cells, and the NLRP3 inflammasome-related genes were determined. Relations between these parameters and MS activity and annualized relapse rate (ARR) were evaluated. Expression of the three lncRNAs, FOXP3, NLRP3, ASC, and caspase-1 along with serum TGF-β1and IL-1β levels were measured in 70 RRMS patients (35 during relapse and 35 in remission) and 30 healthy controls. RRMS patients showed significant downregulation of lnc-EGFR and FOXP3 together with dramatic upregulation of SNHG1, lincRNA-Cox2, NLRP3, ASC and caspase-1 expression compared to controls. Lower serum TGF-β1 and elevated IL-1β levels were observed in RRMS patients. Notably, patients during relapses displayed more significant alterations than those in remission. Lnc-EGFR was positively correlated with FOXP3 and TGF-β1, and negatively correlated with ARR, SNHG1, lincRNA-Cox2 and NLRP3 inflammasome-components. Meanwhile, SNHG1 and lincRNA-Cox2 were positively correlated with ARR, NLRP3, ASC, caspase-1 and IL-1β. Excellent diagnostic performance for lnc-EGFR, FOXP3 and TGF-β1 was demonstrated, while all the biomarkers exhibited strong prognostic potential for predicting relapses. Finally, the differential expression of lnc-EGFR, SNHG1, and lincRNA-Cox2 in RRMS patients especially during relapses suggests their involvement in RRMS pathogenesis and activity. Moreover, correlation between their expression and ARR implies relation to disease progression. Our findings also highlight their promising roles as biomarkers for RRMS.
546			_aText in English, abstracts in English and Arabic.
650		0	_aBiochemistry
856	4	0	_3DSpace electronic resources _uhttp://repository.fue.edu.eg/handle/123456789/6248
942			_cTHESIS _2ddc