000			04794ntm a2200397 i 4500
001			12102116
005			20240721160322.0
008			150118s2024 ua a f bm 000 0 eng |
040			_aEG-EULC _cEG-EULC _erda
041	0		_aeng _bara
082	0	4	_222 _a615.321 _bS.S.C
100	1		_aSarah Mohamed Osama Saeed _eauthor
245	1	0	_aChemical and Biological Evaluation of Bioactive Secondary Metabolites Isolated from Endophytic Fungi of Hedera helix L. Plant / _c By Sarah Mohamed Osama Saeed; supervisor Prof. Dr. Moshera M. El-Sherei Professor of Pharmacognosy Faculty of Pharmacy Cairo University, Prof. Dr. Osama M. Salama Late Professor of Pharmacognosy Vice President Future University in Egypt, Prof. Dr. Osama M. Salama Late Professor of Pharmacognosy Vice President Future University in Egypt, Assoc. Prof. Marwa M. Rafaat Associate Professor of Microbiology Faculty of Pharmacy Future University in Egypt, Dr. Mokhtar M. Bishr Technical Director, Plant Manager of Arab Co. For Pharmaceuticals & Medicinal Plants (Mepaco).
246	1	5	_aتقييم كيميائي وبيولوجي للمركبات المفصولة من الفطريات الداخلية لنبات هيدرا هيليكس ل.
264		1	_c2024
300			_a88 pages, III pages : _billustrations ; _c24 cm
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
500			_aUnder the Supervision of Prof. Dr. Moshera M. El-Sherei Professor of harmacognosy Faculty of Pharmacy Cairo University Prof. Dr. Osama M. Salama Late Professor of Pharmacognosy Vice President Future University in Egypt Prof. Dr. Dalia A. M. Al-Mahdy Professor of Pharmacognosy Faculty of Pharmacy Cairo University Pharmacognosy Department Faculty of Pharmacy Cairo University Egypt 2024 Assoc. Prof. Marwa M. Rafaat Associate Professor of Microbiology Faculty of Pharmacy Future University in Egypt Dr. Mokhtar M. Bishr Technical Director, Plant Manager of Arab Co. For Pharmaceuticals & Medicinal Plants (Mepaco)
500			_aFor the Degree of Doctor of Philosophy in Pharmaceutical Sciences (Pharmacognosy).
502			_aMaster Degree of Pharmacognosy - Faculty of Pharmacy- Cairo University 2019.
504			_aIncludes bibliographical references.
520	3		_aThe demand for new and useful compounds to help and relieve people in all aspects of their lives is constantly increasing. Drug resistance in bacteria, the emergence of life-threating viruses, the enormous spreading of cancer, recurring disease problems in organ transplant recipients, and the massive increase in the incidence of fungal infections in the world's population all highlight our inability to deal with those medical issues. Owing to all these facts, there is a need to explore new and useful bioactive compounds to provide assistance and relief in all aspects of human conditions. Thus, the aim of this study was to screen the well-known medicinal plant Hedera helix L. for its endophytic fungi and to investigate their potential antimicrobial activities as well as their L-asparaginase production ability. Bio-guided fractionation approach was conducted to isolate the pure compounds from the most active fungal fraction. Out of total 6 different isolated endophytic fungal strains, only Aspergillus cejpii showed the highest activity against all tested microbial strains. The bio-guided fractionation of the DCM: MeOH fraction led to the isolation of spiculisporic acid. The compound showed good antimicrobial activities with minimum inhibitory concentration (MIC) values ranging from 3.9-31.25 μg/mL against all tested strains. Concerning the L-asparaginase enzyme production, Fusarium solani showed highest L-ASNase production (22.76 IU/ml/hr) and thus was selected for additional characterization and optimization studies on maximizing its productivity in addition to evaluating its subsequent cytotoxicity towards acute monocytic leukemia (THP1) and Human Skin Fibroblast (HSF) cell lines where it revealed promising cytotoxic activity against acute monocytic leukemia with IC50 = 3.66 μg/ml with low cytotoxicity against tested normal Human Skin Fibroblast cell line.
546			_aText in English, abstracts in English and Arabic.
650		0	_aPharmacognosy
650		0	_aMedicinal plants
700	1		_aProf. Dr. El-Sherei, Moshera M. _esupervisor _934078
700	1		_aProf. Dr. Salama, Osama M. _esupervisor _934079
700	1		_aProf. Dr. Al-Mahdy, Dalia A. M. _esupervisor _934080
700	1		_aAssoc. Prof. Rafaat, Marwa M. _esupervisor _934081
700	1		_aDr. Bishr, Mokhtar M. _esupervisor _934082
856	4	0	_3DSpace electronic resource _uhttp://repository.fue.edu.eg/xmlui/handle/123456789/6333
942			_cTHESIS
999			_c13183 _d13183