000 02253nam a22003137i 4500
999 _c1928
_d1928
005 20201223134026.0
008 100906s enk||||g |||| 001 0 eng d
020 _a0521415454
020 _a0521019702
020 _a9780521019705
040 _afue
_erda
082 0 4 _221
_a574.87028
_bW.J.F.
100 1 _aWatson, James V.
_97791
_eAuthor.
245 1 0 _aFlow cytometry data analysis :
_bbasic concepts and statistics /
_cJames V.Watson.
264 1 _aCambridge :
_bCambridge University Press ,
_c1992.
300 _a288 pages. :
_billustrations. ;
_c20 cm.
336 _2rdacontent
_atext
337 _2rdamedia
_aunmediated
338 _2rdacarrier
_avolume
500 _aInclude index.
505 0 _a 1. Introduction; 2. Fundamental concepts; 3. Probability functions; 4. Significance testing and fit criteria; 5. Regression analysis; 6. Flow cytometric sources of variation; 7. Immunofluorescence data; 8. DNA histogram analysis; 9. Cell cycle kinetics; 10. Dynamic cellular events; 11. Multi-variant analysis primer; 12. Epilogue; Appendices; References; Index.
520 _aThis book covers very basic number handling techniques, regression analysis, probability functions, statistical tests and methods of analyzing dynamic processes from flow cytometry data. These are developed for the analysis of not only individual DNA histograms to obtain the proportion of cells in the cell cycle phases, but also time courses of DNA histograms to yield cell cycle kinetic information; overlapping immunofluorescence distributions with confidence limits for the estimated proportions; enzyme kinetic and membrane transport parameters and a brief introduction to multivariate analysis is given. A distinction is made between data handling, for example gating and counting the numbers of cells within that gate, a process commonly regarded as data analysis but which, in reality, is data handling, and data analysis itself which is the means by which information is extracted.
650 4 _aFlow cytometry
_xstatistical methods.
650 4 _aFlow cytometry
_xmathematics.
650 4 _aFlow cytometry
_xdata processing.
856 _3Abstract
_uhttp://repository.fue.edu.eg/xmlui/handle/123456789/1523
942 _2ddc
_cBK