000			03399cam a22003494i 4500
999			_c2013 _d2013
001			4994692
005			20200915105538.0
008			990304s1999 gw a b 001 0 eng
020			_a3540646000 (wireobind : alk. paper)
040			_aDLC _cDLC _dDLC _erda
082	0	0	_a612.670724 _221 _bS
245	0	0	_aStudies of aging / _cHal Sternberg, Paola S. Timiras (eds.).
264		1	_aBerlin ; _aNew York : _bSpringer, _c[1999]
300			_axi, 208 pages : _billustrations ; _c24 cm
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
490	0		_aSpringer lab manual
504			_aIncludes bibliographical references and index.
505	0		_a A Molecular Methods: Introduction to Molecular Methods -- 1 Genotypic Selection of Age-Related DNA Rearrangements by PCR -- 2 Measurement of Antioxidants in Cutaneous Tissues -- B Cellular Methods: Introduction to Cellular Methods -- 3 Cellular Aging / Replicative Senescence -- 4 Methods for Evaluating a Slice Culture Model of Alzheimer's Disease -- 5 Cell Death -- 6 Method for Studying the Migration of Aging Vascular Smooth Muscle Cells -- 7 Sensitized Chemiluminescence and Fluorescence Methods in Studies of Oxidative Stress -- C Animal Models: Introduction to Animal Models -- 8 How to Choose An Animal Model -- 9 Measuring Mortality and Reproduction in Large Cohorts of the Mediterranean Fruit Fly -- D Physiological Methods: Introduction to Physiological Methods -- 10 Methods for Evaluating in Vivo Rodent Models for Alzheimer's Disease -- 11 Dietary Restriction in Aging -- 12 Methods for the Evaluation of the Hypothalamo-Pituitary-Adrenoccortical Axis in the Aged Rat -- 13 Assessing Blood Replacement Fluids for Organ Preservation and Low Temperature Surgery -- 14 Brain Imaging in Normal and in Alzheimer's Disease.
520			_aInterest in the study of the biology of aging especially as it relates to humans has increased steadily in the last 50 years and continues today to be at the forefront of experimental biology. Due to the many recent advances in this area selection of the best experimental approach may be difficult. It is the purpose of this laboratory manual to provide the researcher with a specific group of techniques with emphasis on hands-on information. A variety of protocols using molecular, cellular and physiological techniques are presented in detail and appropriate animal models have been selected for the study of aging and aging-related diseases. Non-invasive in vivo techniques are described for the study of Alzheimer's dementia and cardiovascular diseases. Techniques for detection of oxidative processes are presented to explore the role of free radicals in causing cell damage and mutations as well as techniques to study apoptosis for its role in cell aging.
650		0	_aAging _xResearch _xMethodology.
650		0	_aOlder people _xDiseases _xResearch _xMethodology.
650		0	_aGeriatrics _xMethodology.
700	1		_aSternberg, Hal, _d1953-, _98168 _eeditor.
700	1		_aTimiras, Paola S., _d1923-, _98169 _eeditor.
906			_a7 _bcbc _corignew _d1 _eocip _f19 _gy-gencatlg
942			_2ddc _cBK