000			03278cam a22003854i 4500
999			_c2021 _d2021
001			4049914
005			20200914091630.0
008			990706s2000 enka b 001 0 eng
020			_a0521620430 (hardback)
020			_a0521629691 (pbk.)
040			_aEG-NcFUE _cEG-NcFUE _dEG-NcFUE _erda
082	0	0	_a612.41 _221 _bH
245	0	0	_aHaematopoietic and lymphoid cell culture / _cedited by Margaret J. Dallman and Jonathan R. Lamb.
264		1	_aCambridge, U.K. ; _aNew York : _bCambridge University Press, _c2000.
300			_axiii, 188 pages : _billustrations ; _c23 cm
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
490	0		_aHandbooks in practical animal cell biology
504			_aIncludes bibliographical references and index.
505	0		_aList of contributors; Preface; Introduction; 1. Haematopoietic differentiation of embryonic stem cells John Ansell and Nicholas Hole; 2. Dendritic cells Jonathan M. Austyn, David Chao, Chen-Lung Lin, Justin A. Roake and Rakesh Suri; 3. Murine thymic explant cultures Kim L. Anderson and John J. T. Owen; 4. T cells Hergen Spits and Colin G. Brooks; 5. B lymphocytes Anne E. Corcoran and Ashok R. Venkitaraman; 6. Monocytes and macrophages James A. Mahoney, Richard Haworth and Siamon Gordon; 7. NK cells and LAK cells Colin G. Brooks and Hergen Spits; 8. Mast cells and basophils Gunnar Nilsson and Dean D. Metcalfe; Index.
520			_ahis invaluable reference handbook describes the fundamental principles and procedures underlying the successful isolation of viable, functionally intact haematopoietic and lymphoid cells, and their maintenance as primary cultures. It provides the technical information on the signals and mediators required for the differentiation and growth of these cells, and is designed for laboratory investigators with limited practical experience in cell culture. There are chapters on dendritic cells, T and B lymphocytes, monocytes and macrophages, NK and LAK cells, mast cells and basophils, as well as on haematopoietic differentiation of embryonal stem cells and on culturing murine thymic explants. Each chapter has been written by experts who have direct practical experience of the techniques and can therefore provide tips for the avoidance of common pitfalls, as well as an insight into the fundamental questions in cell biology and immunology which can be addressed using each cell culture model.
650		0	_aHematopoietic stem cells _vHandbooks, manuals, etc.
650		0	_aImmunocompetent cells _vHandbooks, manuals, etc.
650		0	_aCell culture _vHandbooks, manuals, etc.
700	1		_aDallman, Margaret J., _d1957- _98207
700	1		_aLamb, Jonathan R. _98208
856	4	1	_3Sample text _uhttp://www.loc.gov/catdir/samples/cam032/99038930.html
856	4	2	_3Publisher description _uhttp://www.loc.gov/catdir/description/cam0210/99038930.html
856	4	1	_3Table of contents _uhttp://www.loc.gov/catdir/toc/cam026/99038930.html
906			_a7 _bcbc _corignew _d1 _eocip _f19 _gy-gencatlg
942			_2ddc _cBK