000			05940cam a22003977a 4500
999			_c9307 _d9307
001			17598920
005			20190512130642.0
008			130123s2013 nyua b 001 0 eng d
010			_a 2013931173
020			_a9781627032834 (alk. paper)
020			_a1627032835 (alk. paper)
020			_z9781627032841 (eBook)
035			_a(OCoLC)ocn812252334
040			_aBTCTA _beng _cBTCTA _dRML _dOCLCO _dYDXCP _dEYM _dDLC _erda
042			_alccopycat
050	0	0	_aQH450 _b.G4629 2013
082	0	4	_222 _a572.865 _bG
245	0	0	_aGene regulation : _bmethods and protocols / _cedited by Minou Bina.
260			_aNew York : _bHumana Press ; _bSpringer, _cc2013.
300			_axv, 401 p. : _bill. (some col.) ; _c26 cm.
336			_2rdacontent _atext
337			_2rdamedia _aunmediated
338			_2rdacarrier _avolume
490	1		_aMethods in molecular biology ; _vv. 977
504			_aIncludes bibliographical references and index.
505	0	0	_tGene regulation / _rMinou Bina -- _tIsolation of nuclei for use in genome-wide DNase hypersensitivity assays to probe chromatin structure / _rGuoyu Ling and David J. Waxman -- _tDNase I digestion of isolated nulcei for genome-wide mapping of DNase hypersensitivity sites in chromatin / _rGuoyu Ling and David J. Waxman -- _tIsolation and analysis of DNA derived from nucleosome-free regions / _rMatthew Murtha ... [et al.] -- _tAcquisition of high quality DNA for massive parallel sequencing by in vivo chromatin immunoprecipitation / _rM. van den Boogaard ... [et al.] -- _tLuciferase assay to study the activity of a cloned promoter DNA fragment / _rNina Solberg and Stefan Krauss -- _tPromoter deletion analysis using a dual-luciferase reporter system / _rYong Zhong Xu ... [et al.] -- _tApplication of mRNA display for in vitro selection of DNA-binding transcription factor complexes / _rSeiji Tateyama and Hiroshi Yanagawa -- _tIsolation of intracellular protein - DNA complexes using HaloCHIP an antibody-free alternative to chromatin immunoprecipitation / _rDanette L. Daniels and Marjeta Urh -- _tModified yeast one-hybrid system for genome-wide identification of transcription factor binding sites / _rKazuyuki Yanai -- _tIdentifying specific protein-DNA interactions using SILAC-based quantitative proteomics / _rCornelia G. Spruijt, H. Irem Baymaz, and Michiel Vermeulen -- _tElectrophoretic mobility-shift and super-shift assays for studies and characterization of protein-DNA complexes / _rElsie I. Pares-Matos -- _tCombination of native and denaturing PAGE for the detection of protein binding regions in long fragments of genomic DNA / _rKristel Kaer and Mart Speek -- _tQuantitative nanoproteomics approach for protein complex (QNanoPX) using gold nanoparticle-based DNA probe / _rShu-Hui Chen and Mei-Yin Lin -- _tChromatin assembly and in vitro transcription analyses for evaluation of individual protein activities in multicomponent transcriptional complexes / _rTakayuki Furumatsu and Hiroshi Asahara -- _tUsing FRET to monitor protein-induced DNA bending : the TBP-TATA complex as a model system / _rRebecca H. Blair, James A. Goodrich, and Jennifer F. Kugel -- _tPromoter independent abortive transcription assays unravel functional interactions between TFIIB and RNA polymerase / _rSimone C. Wiesler, Finn Werner, and Robert O.J. Weinzierl -- _tFluorescence cross-correlation spectroscopy (FCCS) to observe dimerization of transcription factors in living cells / _rHisayo Sadamoto and Hideki Muto --- _tNuclear recruitment assay as a tool to validate transcription factor interactions in mammalian cells / _rC.J.J. Boogerd, V.M. Christoffels, and P. Barnett -- _tPreparation of cell lines for single-cell analysis of transcriptional activation dynamics / _rIlona U. Rafalska-Metcalf and Susan M. Janicki -- _tPeptide microarrays for profiling of serine/threonine kinase activity of recombinant kinases and lysates of cells and tissue samples / _rRiet Hilhorst ... [et al.] -- _tImmunoaffinity purification of protein complexes from mammalian cells / _rChieri Tomomori-Sato ... [et al.] -- _tSimple and efficient identification of chromatin modifying complexes and characterization of complex composition / _rJeong-Heon Lee and David Skalnik -- _tHeavy methyl-SILAC labeling coupled with liquid chromatography and high-resolution mass spectrometry to study the dynamics of site-specific histone methylation / _rXing-Jun Cao, Barry M. Zee, and Benjamin A. Garcia -- _tAnalysis of p300 occupancy at the early stage of stem cell differentiation by chromatin immunoprecipitation / _rMelanie Le May and Qiao Li -- _tMammalian two-hybrid assays for studies of interaction of p300 with transcription factors / _rDaniela B. Mendonca, Gustavo Mendonca, and Lyndon F. Cooper -- _tFluorescence anisotropy microplate assay to investigate the interaction of full-length steroid receptor coactivator-1a with steroid receptors / _rChen Zhang, Steven K. Nordeen, and David J. Shapiro -- _tUse of histone deacetylase inhibitors to examine the roles of bromodomain and histone acetylation in p300-dependent gene expression / _rJihong Chen and Qiao Li -- _tHistone deacetylase inhibitor valproic acid as a small molecule inducer to direct the differentiation of pluripotent stem cells / _rJihong Chen, Natascha Lacroix, and Qiao Li -- _tSedimentation and immunoprecipitation assays for analyzing complexes that repress transcription / _rPing Lu ... [et al.] -- _tMethods for studies of protein interactions with different DNA methyltransferases / _rJianchang Yang.
650		0	_aGenetic regulation _vLaboratory manuals.
650		0	_aGene Expression Regulation _vLaboratory Manuals
700	1		_aBina, Minou.
830		0	_aMethods in molecular biology (Clifton, N.J.) ; _vv. 977.
856			_3Abstract _uhttp://repository.fue.edu.eg/xmlui/handle/123456789/2855
906			_a7 _bcbc _ccopycat _d2 _encip _f20 _gy-gencatlg
942			_2ddc _cBK